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IPTG, CAS#:367-93-1
Catalog # | Pkg Size | Price | Quantity | Shopping Cart |
---|---|---|---|---|
R-S-9003 | g | ¥ 800 | 加入購(gòu)物車 |
結(jié)構(gòu)式
外觀 | White powder |
分子量 | 238.31 |
溶解度 | 有機(jī)溶劑或水 |
CAS號(hào) | 367-93-1 |
質(zhì)量控制 | 95% |
儲(chǔ)存條件 | -20℃ |
保存時(shí)間 | 1 Year |
其他信息 | 異丙基1-硫代-β-D-半乳糖吡喃糖苷 |
異丙基1-硫代-β-D-半乳糖吡喃糖苷(IPTG)是β–半乳糖苷酶的活性誘導(dǎo)物質(zhì),常用于藍(lán)白斑篩選及IPTG誘導(dǎo)的細(xì)菌內(nèi)的蛋白表達(dá)等。當(dāng)pUC系列的載體DNA(或其他帶有l(wèi)acZ 基因載體DNA)以lacZ 缺失細(xì)胞為宿主進(jìn)行轉(zhuǎn)化時(shí)、或用M13噬菌體的載體DNA進(jìn)行轉(zhuǎn)染時(shí),如果在平板培養(yǎng)基中加入X–Gal和IPTG,由于β–半乳糖苷酶的α–互補(bǔ)性,可以根據(jù)是否呈現(xiàn)白色菌落(或噬菌斑)而方便地挑選出基因重組體。此外,它還可以作為具有l(wèi)ac或tac等啟動(dòng)子的表達(dá)載體的表達(dá)誘導(dǎo)物使用。
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